Botryosphaeria dieback (BD) caused by Botryosphaeriaceae spp. and Eutypa dieback (ED) caused by Diatrypaceae spp. are two of the most important grapevine trunk diseases in Australian vineyards. Infection often occurs via BD and ED spores landing on open pruning wounds, subsequently colonising the xylem, leading to internal necrosis of the wood, dieback and eventual grapevine death (Gramaje et al. 2018). Prevention of infection is one of the primarily tools grape-growers have for the control of grapevine trunk diseases, however, timing of spore dispersal can vary annually and between different regions (Gramaje et al. 2018). Understanding BD and ED spore dispersal patterns in different grape-growing regions in Australia has been an area of focus in recent years. Previous spore trapping studies reported greater aerial spore dispersal in early spring in South Australia while in New South Wales the summer period had the highest spore dispersal events (Sosnowski et al. 2022). Therefore, a regional approach is required to determine spore dispersal patterns in other Australian grape-growing regions. Burkard spore traps were deployed in the Coal River Valley, Tasmania, in the Yarra Valley, Victoria and in Margaret River, Western Australia. Aerial spore numbers, local minimum and maximum temperatures and rainfall were recorded every 2 days with spore trap tapes collected monthly in 2022 and 2023. Each 2-day period, spore counts were calculated for BD and ED by extracting DNA from 2-day sections of tape and conducting qPCR using Botryosphaeriaceae spp. and Diatrypaceae spp. specific assays (Sosnowski et al. 2017). As the diversity of Diatrypaceae species varies between different grape-growing regions in South Australia and New South Wales (Billones-Baaijens et al. 2023), spore tape samples positive for ED spores were further analysed using high resolution melting analysis to further identify which ED pathogens were present in each grape-growing region for the current study. Rainfall appears to be the primary environmental factor influencing detection of BD and ED aerial spore dispersal in these regions in accordance with previous findings (Sosnowski et al. 2022). However, other weather data was only weakly correlated and further data collection from Australian vineyards may aid in further predicting spore dispersal for BD and ED pathogens. Data collection from these regions continues to further enhance our understanding of the annual variation in aerial spore dispersal. This data will help grape-growers make informed decisions about timing of pruning and wound protection application in different grape-growing regions in Australia.