A Survey was conducted during July 2014 to August 2015 to record disease incidence of root rot in Guar. The study area was based on 52 sites of 13 places from five districts of Sindh. i.e., Mirpurkhas, Hyderabad, Tando Allahyar, Sanghar and Karachi. Fusarium spp. was the most common destructive root rot fungi found in all survey areas. The colonization percentage of Fusarium spp. was recorded to be between 55 to be 100%. Four different species of Fusarium; namely Fusarium solani, F. oxysporum, F. moniliforme, F. semitectum were isolated from the sampled areas. Another root infecting fungi Macrophomina phaseolina, was found that ranged between 0 – 45 %. The 3rd tested root rot pathogen, Rhizoctonia solani was found at its lowest distribution (0 - 30 %) in all governorates on the diseased, survey area. These results showed that Fusarium spp. is the most prevalent in the soil of the agricultural field and responsible for the decline of guar crop. In this study, physicochemical properties of soil i.e. soil organic matter, Ec, cation and an-ion showed significant variation and K+ deficiency was recorded in all sampled areas