The recent detection of rice blast (Magnaporthe oryzae syn. Pyricularia oryzae) in the Northern Rivers region of New South Wales (NSW) marks a concerning new development for Australia’s rice industry. This first report of rice blast in the region is accompanied by the isolation and molecular characterisation of several isolates, which were assessed for pathogenicity and genetic diversity. Symptoms typical of rice blast (lesions on leaves, panicles, and stems) were observed during the 2024 growing season, prompting immediate investigation. Isolates were characterised through morphological analysis, molecular techniques, and pathogenicity testing, revealing a range of virulence and genetic variability among the collected strains. The potential spread of rice blast to the Riverina region, the heart of Australia’s rice production, could have devastating consequences, leading to severe yield losses and threatening the viability of the industry. The introduction of this pathogen underscores the urgent need for enhanced surveillance, targeted management strategies, and resistance breeding efforts to mitigate the risk of a broader epidemic. This study provides critical baseline data for ongoing research into the dynamics of rice blast in Australia and its management in the face of potential regional expansion.